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KMID : 1041219960380010013
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Journal of Sericultural and Entomological Science
1996 Volume.38 No. 1 p.13 ~ p.18
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Construction of the cDNA Library from Bombyx mori Larvae and Analysis of the Partial cDNA Sequences
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김상현/Kim, Sang Hyun
윤은영/강석우/김근영/강석권/Yun, Eun Young/Kang, Seok Woo/Kim, Keun Young/Kang, Seok Kwon
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Abstract
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To secure the genetic resources of silkworm, Bombyx mori, the cDNA library was constructed with mRNA isolated from fifth instar larvae. Titer of the cDNA library was about1.3 X 106 plaques in total. We presumed that the titer covered all transcripts existed in Bombyx mori. Meanwhile, it is known that partial cDNA sequences, Expressed Sequence Tags (ESTs), have a good value for the discovery of novel genes and the elucidation of their structures. For this purpose, partial cDNA sequencing was carried out from randomly selected cDNA clones in the library. Partial cDNA sequences of 37 clones were determined and an average of 212 nucleotides of sequence can be read from the clone. The ESTs were searched in GenBank database and fifteen ESTs showed significant similarities to enlisted sequences. They included the genes of storage protein, heat shock protein, actin, catalase and so forth. We presumed that the 22 unmatched ESTs were novel genes.
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